It seems the plug in works only in imgaj 1.29. I believe that, although quite some time has already passed since then, may know something about it. Since in the discussion I mentioned, the possibility of fixing this issue with Punta Analyzer was considered, maybe by developing a Puncta-Analyzer v2, I was wondering whether something was advanced in this sense. I then ran the plugin in an older version of ImageJ (1.46r Java 1.8.0_66), only to obtain the same exception: : ij.ImagePlus.getMask()[IĪt Puncta_Analyzer$ntractHistogram(Puncta_Analyzer.java:708)Īt Puncta_Analyzer.locatePunctaInColorBand(Puncta_Analyzer.java:660)Īt Puncta_Analyzer.locatePuncta(Puncta_Analyzer.java:620)Īt Puncta_n(Puncta_Analyzer.java:240)Īt ij.(PlugInFilterRunner.java:256)Īt ij.(PlugInFilterRunner.java:105)Īt ij.nCommand(Executer.java:127) I’ve managed to install Puncta-Analyzer v1.1 in Fiji-ImageJ (ImageJ v1.51a Java 1.8.0_66), but couldn’t get it to show nor save any results. However, I’m experiencing difficulties with it, and these are the same that a coupled of years ago started a discussion in ImageJ’s mailing list In order to do so, I was told to try the ImageJ plugin “Puncta Analyzer”. The class ij.IJ is a convenience class with many static functions.I need to analyse some fluorescent confocal images stained with double presynaptic markers and other images stained with pre- and postsynaptic markers. An overview of all the javadoc resources can be found on this page with javadoc links. The source of the various Fiji-related projects is spread over several source code repositories, and so is their API documentation. … and repeat until your plugin is working as intended.
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